Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Acetaminophen causes neurodevelopmental injury in susceptible babies and children: no valid rationale for controversy Tylenol's Impact on Glutathione and Liver Damage Tylenol, Autism, And The Glutathione Connection OC Integrative Medicine