Frontiers Potential adverse actions of prenatal exposure of acetaminophen to offspring The Metabolism of Acetaminophen and the Synthesis of Glutathione Download Scientific Diagram Glutathione Depletion in Mitochondrial Diseases Glutathione Reporter Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.