Cysteine conjugates of acetaminophen and p aminophenol are potent inducers of cellular impairment in human proximal tubular kidney HK 2 cells Archives of Toxicology Springer Nature Link Acetaminophen Toxicity: How Genetic Susceptibility Combines with Environmental Factors Figure 1: CMAJ Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.