View of Acetaminophen, the missing anion gap in the patient with metabolic acidosis The Southwest journal of medicine Combination of Paracetamol and the Glutathione Depleting Agent Buthionine Sulfoximine Show Differential Effect on Liver Cancer Cells and Normal Hepatocytes Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. A study published in Gastroenterology describes a new mitochondrial mediator in the hepatotoxicity induced by paracetamol. Institute of Biomedical Research of Barcelona