Tylenol depletes glutathione levels Is Tylenol Safe During Pregnancy? Understanding Risk Factors Seeking Health Serotonin Deficiency Exacerbates Acetaminophen Induced Liver Toxicity In Mice Scientific Reports Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.