Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Tylenol depletes glutathione your liver's most important protective antioxidant. Every dose chips away at it. So instead of taking lower doses every 46 hours, I recommend spacing to 1000mg every 8 hours. How Dangerous is Tylenol? Acetaminophen induces liver injury and depletes glutathione in mice brain: Prevention by Moringa oleifera extract ScienceDirect