Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Better alternatives for pain relief than Tylenol NSAIDs Turnpaugh Health & Wellness Frontiers Potential adverse actions of prenatal exposure of acetaminophen to offspring Tylenol depletes glutathione your liver's most important protective antioxidant. Every dose chips away at it. So instead of taking lower doses every 46 hours, I recommend spacing to 1000mg every 8 hours.