Tylenol And Glutathione Invigor Medical Glutamine Vs Glutamate: What's The Difference? Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Association of antioxidant nutraceuticals and acetaminophen (paracetamol): Friend or foe? ScienceDirect