Activation and Transportation of Fatty Acids to the Mitochondria via the Carnitine Shuttle with Animation Animations PharmaXChange.info Role of Carnitine Shuttle System in the Active Transport of Fatty Acyl Coa into the mitochondrial L Carnitine functions as a cofactor for long chain fatty acids across Download Scientific Diagram Short Note On Carnitine Shuttle: Fatty acids are oxidized in mitochondria, and synthesized in the cytoplasm. Long chain fatty acids must form an active intermediate, fatty acyl CoA, before being oxidized inside mitochondria. Catherine